20
1. INTRODUCTION
solution. For example, an electric eld of 25 V/µm is required to overcome the inu-
ence of PDMS insulation layer.
4. Photolithography mold method, a nano-conductive material (nano-carbon powder or
nano-silver powder) is mixed with PDMS, and the mixture is plastered to a photoli-
thography mold, and then use blade to scrape and form a pattern, which is embedded
into the PDMS microchannel [145]. Lewpiriyawong et al. prepared a thick-electrode
chip using a mixture of nano-silver powder and PDMS (AgPDMS) to achieve separa-
tion of particles and cells [146]. Based on the same processing technology, Marchalot
et al. used a mixture of nano-conductive carbon powder and PDMS (C-PDMS) to
make a thick-electrode chip to achieve cell enrichment [147]. is PDMS conductive
mixture retains the properties of PDMS, which can be easily bonded to the substrate.
e thick electrodes have high conductivity, ductility and repeatability.
1.4 RESEARCH PURPOSES AND SIGNIFICANCES
Biomanipulation based on DEP technology is currently a research hotspot in academia. In order
to overcome the limitations of 2D electrodes, the thick-electrode structures are adopted to design
single-cell operation chip, especially to solve the problem of single-cell 3D rotation. First, by con-
structing a multi-electrode structure with thick electrodes to realize single-cell 3D electro-rotation,
electrical parameter measurement and morphology imaging. is work can demonstrate the value
of thick-electrode DEP multi-electrode structure in the eld of single-cell manipulation. On this
basis, the thick-electrode DEP multi-electrode structure is expanded by opto-electronic integra-
tion, and dual optical bers are embedded in the thick electrodes, which enable the chip to rotate
and stretch single cells, achieving multi-parameter measurement of mechanical and electrical prop-
erties of single cells, extends the application of thick-electrode DEP in biological manipulation
and analysis.
1.5 MAIN CONTENT OF THE BOOK
Chapter 1: Introduction. e background and signicance of the topic. First, the background and
current situation of microuidic development are briey summarized. e materials and processing
methods of the microuidic chip were analyzed, and the typical biological manipulation mecha-
nisms are compared according to the technical means.
e research progress of single-cell manipulation by DEP is introduced. en the research
status of electrode processing in DEP technology is analyzed, and the method basis is provided
for the design of chip structure. Finally, the purpose and signicance of this research are proposed
according to the requirements of biological applications.
21
Chapter 2: ick-electrode DEP for single-cell 3D rotation. A thick-electrode DEP
multi-electrode chip was proposed for single-cell 3D electro-rotation. First, the thick-electrode
multi-electrode chip structure is presented. e working principle and design idea of the chip are
introduced, and the feasibility of 3D rotation structure based on multiple thick electrodes is veried
by simulation analysis, then set up the experimental platform. Based on the rotation platform, 3D
electrorotation of single cells is demonstrated, and single-cell electrical parameters and physical
topography parameters are measured. e work revolves around the application of multi-electrode
structure of thick-electrode DEP in single-cell 3D rotation, which extends the application of
thick-electrode DEP.
Chapter 3: Opto-electronic integration of thick-electrode DEP microuidic chip. By
extending the function of the multi-electrode chip with thick-electrode DEP, the ber optical
stretcher is embedded into the thick electrodes to stretch and rotate the single cell. First, the
application requirement of single-cell multi-parameter measurement for thick-electrode DEP
optoelectronic integrated chip is introduced. en, the principle of single-cell optical stretcher and
the analysis method of mechanical properties are analyzed. Subsequently, the thick-electrode DEP
opto-electronic integrated chip is presented, which achieves mechanical and electrical property
measurements of single cells. e work of this chapter focuses on the application of thick-electrode
DEP opto-electronic integrated structure in single-cell multi-parameter measurement, and further
broadens the application of thick-electrode DEP.
Chapter 4: Summary.
1.4 RESEARCH PURPOSES AND SIGNIFICANCES
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