ix
Sensory Organ Replacement and Repair
Gerald E. Miller
2006
Articial Organs
Gerald E. Miller
2006
Signal Processing of Random Physiological Signals
Charles S. Lessard
2006
Image and Signal Processing for Networked E-Health Applications
Ilias G. Maglogiannis, Kostas Karpouzis, and Manolis Wallace
2006
Copyright © 2020 by Morgan & Claypool
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in
any form or by any means—electronic, mechanical, photocopy, recording, or any other except for brief quota-
tions in printed reviews, without the prior permission of the publisher.
3D Electro-Rotation of Single Cells
Liang Huang and Wenhui Wang
www.morganclaypool.com
ISBN: 9781681736914 Paperback
ISBN: 9781681736921 eBook
ISBN: 9781681736938 Hardcover
DOI 10.2200/S00958ED1V01Y201910BME058
A Publication in the Morgan & Claypool Publishers series
SYNTHESIS LECTURES ON BIOMEDICAL ENGINEERING
Lecture #58
Series Editor: John D. Enderle, University of Connecticut
Series ISSN 1930-0328 Print 1930-0336 Electronic
3D Electro-Rotation of
Single Cells
Liang Huang, Wenhui Wang*
State Key Laboratory of Precision Measurement Technology and Instrument
Department of Precision Instrument, Tsinghua University, Beijing, China
*Corresponding author: [email protected]
SYNTHESIS LECTURES ON BIOMEDICAL ENGINEERING #58
M
&C
MORGAN
&
CLAYPOOL PUBLISHERS
xii
ABSTRACT
Dielectrophoresis microuidic chips have been widely used in various biological applications due
to their advantages of convenient operation, high throughput, and low cost. However, most of
the DEP microuidic chips are based on 2D planar electrodes which have some limitations, such
as electric eld attenuation, small eective working regions, and weak DEP forces. In order to
overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were
designed to realize manipulation and multi-parameter measurement of single cells.
Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-ro-
tation chip of “Armillary Sphere” was designed. e chip uses four thick electrodes and a bottom
planar electrode to form an electric eld chamber, which can control 3D rotation of single cells
under dierent electric signal congurations. Electrical property measurement and 3D image re-
construction of single cells are achieved based on single-cell 3D rotation. is work overcomes the
limitations of 2D planar electrodes and eectively solves the problem of unstable spatial position
of single-cell samples, and provides a new platform for single-cell analysis.
Based on multi-electrode structure of thick-electrode DEP, a microuidic chip with opto-
electronic integration was presented. A dual-ber optical stretcher embedded in thick electrodes
can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretch-
ing and rotation manipulation gives the chip the ability to simultaneously measure mechanical and
electrical properties of single cells, providing a versatile platform for single-cell analysis, further
extending the application of thick-electrode DEP in biological manipulation and analysis.
KEYWORDS
thick electrodes, DEP, 3D rotation, optical stretcher, multi-parameter measurement
xiii
Contents
Acknowledgments ........................................................ xvii
1 Introduction ......................................................... . 1
1.1 Overview of Microuidics ........................................ 1
1.1.1 Background and Brief Development History ................... 1
1.1.2 Microuidic Chip Material and Processing Method ............. 2
1.2 Sample Manipulation Methods in Microuidic Chips .................. 5
1.2.1 Fluidic Methods ......................................... 5
1.2.2 Optical Methods ......................................... 7
1.2.3 Magnetic Methods ....................................... 8
1.2.4 Acoustic Methods ........................................ 9
1.2.5 DEP Methods ......................................... 10
1.3 DEP Microuidic Chips ........................................ 12
1.3.1 eory of DEP ......................................... 12
1.3.2 DEP Parameter Analysis ................................. 15
1.3.3 Advances in DEP-Based Single-Cell Manipulation ............ 15
1.3.4 Electrode Fabrication of DEP Chips ........................ 17
1.4 Research Purposes and Signicances ............................... 20
1.5 Main Content of the Book ...................................... 20
2 ick-Electrode DEP for Single-Cell 3D Rotation ......................... 23
2.1 Introduction .................................................. 23
2.2 Progress in Cell Rotation Manipulation ............................ 24
2.3 ick-Electrode Multi-Electrode Chip Design ....................... 27
2.3.1 Principle and Design of ick-Electrode Multi-Electrode
Construction ........................................... 27
2.3.2 Design and Simulation of 3D Rotational Structure of “Armillary
Sphere” ............................................... 30
2.4 Chip Fabrication .............................................. 36
2.5 Experimental Setup ............................................ 38
2.5.1 Experimental Equipment ................................. 38
2.5.2 Signal Conguration ..................................... 38
..................Content has been hidden....................
You can't read the all page of ebook, please click here login for view all page.