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3. OPTO-ELECTRONIC INTEGRATION OF THICK-ELECTRODE DEP MICROFLUIDIC CHIP
3.5 EXPERIMENTAL SETUP
3.5.1 EXPERIMENTAL INSTRUMENTS
Equipment about optical stretcher
1. Single-mode ber (HI1060, Corning)
HI1060 single-mode ber with a diameter of 125 µm.
2. 980 nm pump laser (VENUS-980-M, Shanghai Haoyu)
A pump laser with a wavelength of 980 nm is used, as shown in Figure 3.8(a), and the
power adjustment range is from 0–850 mW. e 980-nm laser wavelength is in the
near-infrared wavelength region, and the absorption rate of the light source in water
is relatively low, so the damage to cells is negligible.
(a) (b)
Figure 3.8: Dual-ber stretcher optical path device: (a) 980-nm pump laser; and (b) 980-nm optocou-
pler.
1. 980-nm optical coupler (VENUS-980-M, Shanghai Haoyu)
As shown in Figure 3.8(b), the optocoupler splits the laser from the laser into two
beams, with a split ratio of 50/50, and the two beams are connected to two sin-
gle-mode bers.
2. Optical isolator (OI-980, Shanghai Haoyu)
e optical isolator is used to prevent laser propagating from one of the opposite
bers from returning into the laser through another optical ber to damage the laser.
e optical isolator, as shown in Figure 3.9(a), is connected between the output of
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the laser and the optical wavelength division multiplexer, which has a small forward
transmission loss to the 980-nm laser and a large reverse transmission loss.
3. Filter mirror (YZ-532LGP-Y68, Yizheng Laser)
e 980-nm laser easily causes the pixels on the CCD camera to be overexposed and
appear as white spots. In order to facilitate the observation of the response of the cells,
a 980-nm lter mirror is placed in front of the CCD camera. Figure 3.9(b) is photo
of a 980-nm lter mirror.
(a) (b)
Figure 3.9: (a) 980-nm laser isolators; and (b) 980-nm lter mirror.
e working diagram and setup of the experiment are shown in Figure 3.10. e chip is
placed under an inverted microscope (Nikon Ti-U) and the four electrodes of the chip are con-
nected to a four-channel signal generator (TGA-12104, TTi). e dual bers are passed through
an optocoupler that is connected to a 980-nm pump laser. e inlet and outlet are led out through
a Teon tube. In this experiment, gravity is used to drive the ow, and the container of cell solution
is placed on the 3D manipulator (MP285, Sutter) which can move up and down to change the
height of the container, and the movement precision can reach 40 nm. Experimental image and
video capture are performed using an industrial camera (acA 640-120 gm, Basler).
3.5 EXPERIMENTAL SETUP
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