71
When the misalignment occurs in the vertical direction, the torque is in the vertical direction,
causing the cells to do out-of-plane rotation. Figure 3.15 shows a MCF7 cell in the vertical direc-
tion misaligned by the dual bers (laser power 200 mW) doing out-of-plane rotation.
(a)
(b)
L
r
cell
Figure 3.15: Dual bers that are misaligned in the vertical direction causes the cells to do out-of-plane
rotation: (a) schematic diagram of the principle: and (b) one MCF7 cell did out-of-plane rotation
under the action of dual bers (laser power 200 mW).
3.6.3 SINGLECELL DUALFIBER CAPTURE EXPERIMENT
When a cell ows through dual bers, the gradient force generated by the laser on the cell pulls the
cell toward the position where the intensity distribution is greatest, that is the center of the axis of
the ber. When the cell is o center of the axis, the gradient force pulls the cell back to the center
of the axis. e axial force generated on the cell pushes the cell away from the ber end. Because
the two bers are relatively distributed, a force balance is formed between the two bers. A single
cell can be stably captured by the combination of gradient force and axial force.
Figure 3.16: e process of capturing one HeLa cell by dual bers.
3.6 SINGLECELL MANIPULATION AND MULTIPARAMETER ANALYSIS
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