77
and the maximum cytoplasmic conductivity; the average cell area-specic membrane capacitance
of MCF7 is about 1.5 times that of MCF10A.
600
300
0
-300
-600
-900
0.6
0.5
0.4
0.3
0.2
0.1
0
10
4
10
6
10
8
10 12 14 16
AC Frequency (Hz) Area-Specic Membrane Capacitance C
mem
(mF/m
2
)
Rotation Speed (°/sec)
Conducitivity of Cytoplasm: σ
cyto
(mS/m)
HeLa
A549
HepaRG
MCF7
MCF10A
HeLa
A549
HepaRG
MCF7
MCF10A
(a) (b)
Figure 3.24: (a) Electro-rotation spectra and tting results of ve types of cells; and (b) distribution of
electrical parameters corresponding to ve types of cells.
Table 3.2: Electrical parameters of ve types of cells
Cell
Types
Area-Specic Membrane Capacitance
C
mem
(mF/m
2
)
Cytoplasmic Conductivity
σ
cyto
(S/m)
HeLa 13.23 ± 0.32 0.35 ± 0.06
A549 17.12 ± 0.14 0.23 ± 0.04
HepaRG 14.71 ± 0.15 0.23 ± 0.07
MCF7 14.15 ± 0.09 0.50 ± 0.03
MCF10A 10.16 ± 0.07 0.55 ± 0.02
3.7 SUMMARY
is chapter is the extension of the function of multi-electrode chips for thick-electrode DEP.
Based on the thick-electrode DEP multi-electrode 3D rotation chip, the thick-electrode DEP
multi-electrode structure integrated with the optical stretcher is presented to achieve multiple
physical parameter measurements of single cells. e single cell was rst captured using an optical
stretcher and the mechanical properties of the cell were measured by step-stress experiments. e
optical stretcher provides a stable spatial position via single-cell capture for single-cell rotation,
which not only ensures the stability of cell electro-rotation but also improves the accuracy of mea-
3.7 SUMMARY
78
3. OPTO-ELECTRONIC INTEGRATION OF THICK-ELECTRODE DEP MICROFLUIDIC CHIP
surement of electrical parameters. e experiment results show that the ve types of cells have dif-
ferent mechanical and electrical properties. e use of this opto-electronic integration chip expands
the application of thick-electrode DEP.
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