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CHAPTER 4
Summary and Outlook
4.1 MAIN WORK
Based on the basic structure of thick-electrode DEP, the multi-electrode composition of thick-elec-
trode DEP was studied. A single-cell 3D rotation chip of “Armillary Sphere” was proposed and
fabricated. e chip design combined with single-cell capture V-shape structure. e trap structure
and the 3D rotation thick-electrode structure solve the problem of single-cell loading diculty.
Single-cell 3D rotation is achieved by applying dierent electrical signal congurations on the elec-
trodes. Based on single-cell 3D rotation manipulation, single-cell electrical parameter measurement
and 3D morphology reconstruction are realized. e reconstructed 3D morphology can be further
used to extract the physical features of single cells, such as surface area, volume, and ellipsoid rate,
providing a new operating platform for single-cell analysis.
To realize the opto-electronic integration application of multi-electrode structure with
thick-electrode DEP, a single-cell optical stretcher and electro-rotation chip was presented. On the
basis of the single-cell electro-rotation chip, a dual-ber optical stretcher was integrated for single
cell capture and mechanical property measurement. e high-eciency single-cell capture ensures
the stable spatial position of the cells, and combined with electro-rotation, can perform single-cell
mechanical and electrical characteristic parameters measurement on the same chip. Five types of
cells were measured in the experiment. e combination of mechanical and electrical properties
conrmed the specicity of single cells, further broadening the application of thick-electrode DEP
in microuidics.
4.2 MAJOR INNOVATIONS
1. Proposed and prepared a single-cell 3D rotation chip with structure resembling “Ar-
millary Sphere,” which overcomes the limitation of electric eld of planar electrodes,
realizes single-cell 3D rotation, electrical parameter measurement, and 3D image
reconstruction.
2. Presented a single-cell optical stretcher-electro-rotation chip with dual bers em-
bedded into thick electrodes to simultaneously measure single-cell mechanical and
electrical properties, providing a versatile operating platform for single-cell studies.
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