xiv
2.5.3 Experimental Methods ................................... 38
2.5.4 3D Rotation Speed Measurement Method ................... 39
2.6 Single-Cell 3D Rotation Experiment .............................. 40
2.6.1 Cell Sample Preparation .................................. 40
2.6.2 Cell Capture Validation .................................. 40
2.6.3 3D Rotation Experiment ................................. 41
2.6.4 Relationship Between Speed and Electrical Signal Parameters . . . . 46
2.7 Cellular Electrical Property Analysis ............................... 48
2.7.1 Principles of Cellular Electrical Parameter Measurement ........ 48
2.7.2 Key Factors in Electrical Property Analysis ................... 50
2.7.3 Experimental Analysis ................................... 52
2.8 Cell 3D Morphology Reconstruction .............................. 53
2.8.1 Principle of Reconstruction ............................... 53
2.8.2 Analysis of Reconstruction Results .......................... 54
2.9 Summary .................................................... 54
3 Opto-Electronic Integration of ick-Electrode DEP Microuidic Chip ....... 57
3.1 Introduction .................................................. 57
3.2 Progress in Single-Cell Mechanical Property Measurement ............. 58
3.3 Electro-Rotation Chip Function Expansion ......................... 60
3.3.1 Electro-Rotation Chip Function Expansion Requirements ....... 60
3.3.2 Principle of Optical Stretcher .............................. 61
3.3.3 Step-Stress Analysis of Cell Mechanical Properties ............. 63
3.4 Chip Design and Fabrication ..................................... 64
3.5 Experimental Setup ............................................ 66
3.5.1 Experimental Instruments ................................ 66
3.5.2 Experimental Steps ...................................... 68
3.6 Single-Cell Manipulation and Multi-Parameter Analysis Experiments . . . . 69
3.6.1 Experimental Demonstration of Filter Mirror ................. 69
3.6.2 Cell Motions when the Fibers Are Misaligned ................ 70
3.6.3 Single-Cell Dual-Fiber Capture Experiment .................. 71
3.6.4 Single-Cell Optical Stretch Experiment ..................... 72
3.6.5 Single-Cell Mechanical Property Measurement ................ 74
3.6.6 Single-Cell Electro-Rotation .............................. 75
3.7 Summary .................................................... 77
4 Summary and Outlook ................................................ 79
4.1 Main Work .................................................. 79
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