Contents

Preface

1       Chickpea: In the Backdrop Note

1.1            General Account of Pulses and Chickpea

1.2            Botanical Description of Chickpea

1.3            Economic Importance of Chickpea

1.4            Origin, Biosystematics and Cytogenetics

1.5            Case Studies

2       Induced Mutagenesis

2.1            Dose Effect and Genotypic Sensitivity

2.2            Induced Mutagenesis and Chickpea

3       Induced Mutagenesis: Biophysiological Damages and Cytological Abberrations

3.1            Bio-Physiological Damages

3.2            Cytological Aberrations

3.3            Case Study 1

3.3.1         Results

3.3.1.1      Seed Germination

3.3.1.2      Seedling Height

3.3.1.3      NRA, Chlorophyll and Carotenoid Contents

3.3.1.4      Cytological Observations

3.3.1.5      Pollen Fertility

3.3.1.6      Plant Survival

3.3.2         Discussion

4       Chlorophyll Mutations

4.1            Case Study 2

4.1.1         Results

4.1.2         Discussion

5       Mutagenic Effectiveness and Efficiency

5.1            Case Study 3

5.1.1         Results

5.1.2         Discussion

6       Morphological Mutations

6.1            Case Study 4

6.1.1         Results

6.1.1.1      NRA, Chlorophyll and Carotenoid Contents of Morphological Mutants

6.1.2         Discussion

7       Induced Mutagenesis and Quantitative Traits

7.1            Case Study 5

7.1.1         Results

7.1.2         Discussion

8       High Yielding and Productive Mutants

8.1            Productive Mutants: Enhanced Micronutrients

8.2            Productive Mutants: Seed Protein Variants

8.3            Case Study 6

8.3.1         Results

8.3.1.1      Screening and Isolation of High Yielding Mutants

8.3.1.2      NRA, Chlorophyll and Carotenoid Contents

8.3.1.3      Seed Protein and Mineral Element Contents

8.3.1.4      SDS-PAGE

8.3.1.5      Correlation Studies

8.3.2         Discussion

8.3.2.1      Physiological Parameters

8.3.2.2      Mineral Elements Composition

8.3.2.3      Seed Protein Content and SDS-PAGE

8.3.2.4      Correlation Studies

9       Recent Technologies and Future Strategies

9.1            Modern Techniques

9.1.1         High Hydrostatic Pressure (HHP)

9.1.2         Ion Beam Technology (IBT)

9.1.3         Space Breeding

9.1.4         Targeting Induced Local Lesions in Genomes (TILLING)

9.1.5         Endonucleolytic Mutation Analysis by Internal Labelling (EMAIL)

9.2            Future Strategies

References

Appendix

List of Figures

List of Tables

Index

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