Table of Contents
Woodhead Publishing Series in Electronic and Optical Materials
Part I: Technology for DNA and RNA analysis of pathogens
1. Nucleic acid sequencing for characterizing infectious and/or novel agents in complex samples
1.1 Pathogen sequencing and applications in public health and biosecurity
1.2 Next-generation sequencing (NGS) technologies and the sequencing landscape
1.3 Characterization of known pathogens
2.2 Real-time PCR: development and description
2.3 Considerations when developing a real-time PCR assay
2.4 Real-time PCR instrument platforms
3. Isothermal amplification of specific sequences
3.2 Melting temperature (Tm) estimation and categories of isothermal amplification technologies
3.3 Isothermal amplification based on DNA polymerases
3.4 Isothermal amplification based on RNA polymerases
4. Bead array technologies for genetic disease screening and microbial detection
Part II: Lab-on-chip and portable systems for biodetection and analysis
5. Electrochemical detection for biological identification
5.2 Electrochemical techniques for bioanalysis
5.3 Electrochemical biosensors for pathogens
6.2 Conductometry in enzyme catalysis
6.6 Conductometric enzyme biosensors based on inhibition analysis
6.7 Whole cell conductometric biosensors
6.8 DNA-based conductometric biosensors
6.9 Conductometric biosensors for detection of microorganisms
7. Bio-chem-FETs: field effect transistors for biological sensing
7.2 The field effect transistor (FET)
7.3 Chemical compounds and biological units as sensing elements in Bio-chem-FETs
7.4 Nanomaterials and nanoengineering in the design of Bio-chem-FETs
8. Microfluidic devices for rapid identification and characterization of pathogens
8.2 Challenges and technical as well as commercial solutions
8.4 Chip-based analysis of protein-based analytes in microfluidic devices
8.5 Chip-based analysis of nucleic acid-based analytes in microfluidic devices
Part III: Optical systems for biological identification
9. Optical biodetection using receptors and enzymes (porphyrin-incorporated)
9.4 Binding of a receptor to a simulated ‘toxin’
9.5 Binding of the simulated 'toxin' to the receptor
9.6 Binding of a specific antigen diagnostic of cancer to a receptor
10. Overview of terahertz spectral characterization for biological identification
10.6 The problem with a poor convergence of simulation
10.7 Other problems: dissipation time scales
10.8 Statistical model for Escherichia coli DNA sequence
10.9 Component-based model for Escherichia coli cells
10.10 Experimental sub-terahertz spectroscopy of biological molecules and species
10.11 Conclusions and future trends
11. Raman spectroscopy for biological identification
11.2 Experimental methods used to capture intensive variability
11.3 Multivariate spectral analysis methods
11.4 Species-level biological identification results
12. Lidar (Light Detection And Ranging) for biodetection
12.2 The value of early warning
12.3 The essentials of Bio-Lidar
12.8 Conclusions and future trends
Part IV: Sample preparation and mass spectrometry-based biological analysis
13. Electrophoretic approaches to sample collection and preparation for nucleic acids analysis
13.2 Separation parameters for nucleic acids for use in sample preparation
13.3 Electrophoresis using uniform electric fields for sample preparation and analysis
13.4 Electrophoresis using non-uniform electric field gradients for sample preparation and analysis
13.5 Comparison of electrophoretic techniques for sample preparation and contaminant rejection
13.7 Sources of further information and advice
14. Mass spectrometry-based proteomics techniques for biological identification
14.2 Bacterial proteome handling, processing and separation methods
14.3 Sample ionization and introduction for mass spectrometry (MS) analysis
14.4 Mass spectral proteomic methods
14.5 Computational and bioinformatics approaches for data mining and discrimination of microbes
14.7 Analysis of MALDI-MS spectra