When you've discovered unannotated transcripts you may want to see whether they are differentially expressed between experiments. We've already looked at how we might do that with edgeR and DESeq, but one problem is going from an object such as a RangedSummarizedExperiment, comprised of the data and a GRanges object that describes the peak regions, to the internal DESeq object. In this recipe, we'll look at how we can summarise the data in those objects and get them into the correct format.