Index

  1.  
  2. a
  3. accurate temperature determination
  4. adherent cells
  5. advanced MFP heads and holders
  6. advection-diffusion transport equations
  7. affinity-based proteomic assays
  8. alkaline phosphatase
  9. α -amino-3-hydroxy-5-methyl--isoxazolepropionic acid (AMPA)
  10. α -hemolysin
  11. amplification-based nucleic acid analysis
  12. ANSYS CFX
  13. antibody assays
  14. antibody barcode arrays
  15. antigen-specific cell sorting
  16. anti-progesterone receptor (α -PR)
  17. aperture-array probes
  18. aqueous two-phase system (ATPS)
    1. antibody assays
    2. bioreagent patterning
    3. channel-free cell and reagent micropatterning
    4. cell patterning, see cell patterning
    5. collagen microgels
    6. dextran
    7. phase diagrams
    8. polyethylene glycol
    9. polymer solutions
  19. aspiration flow rate
  20. AutoMate Scientific
  21.  
  22. b
  23. bacterial cells
  24. biochemical concentration gradient assays
  25. biopatterning device
  26. biopatterning methods
  27. Biopen
    1. adherent cells
    2. application space
    3. cell zeiosis and ion channel activation
    4. future technology
    5. hydrodynamic confinement
    6. intercellular communication
    7. local superfusion of tissue slices
    8. local temperature adjustment and measurement
    9. single cell analysis
    10. single-cell electroporation
    11. single cell enzymology
    12. single-cell viability test
    13. single muscle fiber physiology
    14. superfusion techniques
    15. technology overview
    16. work on tissue samples
  28. bioreagent patterning
  29. blebs
  30. brain slice culture techniques
  31. bulky MFP platform
  32.  
  33. c
  34. Calcium Green-
  35. cancer stem cells
  36. capillary electrophoresis (CE)
  37. capsaicin receptor
  38. Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone
  39. cell blebbing
  40. cell co-culturing
  41. cell exclusion and cell island patterning
  42. cell handling
  43. cell patterning
    1. bacterial cells
    2. cell co-culturing
    3. cell exclusion and cell island patterning
    4. heterocellular stem cell niche engineering
    5. skin tissue engineering
    6. three-dimensional cellular models
  44. cell-to-cell variability
  45. cellular heterogeneity
  46. cell zeiosis
  47. chemical single-cell poration
  48. chemistrodes
    1. design and fabrication
    2. geometry and operation
    3. intracellular processes
    4. isolation and incubation of cells
    5. laminar-flow devices
    6. mass transfer
    7. non-dimensional groups
    8. on-line analysis
    9. operation
    10. parallel off-line analysis
    11. plug coalescence-limited process
    12. single cell stimulation and response analysis
    13. stimuli plugs
  49. circulating tumor cell (CTC) sorting
  50. closed-channel microfluidics
  51. coaxial double barrel system
  52. collagen microgels
  53. computational fluid dynamics (CFD)
  54. COMSOL Multiphysics
  55. concentration gradient measurement
  56. continuous-flow printing
  57. creeping flow
  58. CV, see cyclic voltammetry (CV)
  59. 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX)
  60. cyclic voltammetry (CV)
  61. cytosolic free Ca2+
  62.  
  63. d
  64. Damköhler number
  65. Deborah number (De)
  66. desorption phase
  67. 3,3'-diaminobenzidine (DAB)
  68. dichlorodiphenyltrichloroethane (DTT)
  69. diffusion
    1. advection-diffusion transport equations
    2. high Péclet number asymptotic solutions
    3. model accuracy
  70. dipolar and quadrupolar MFP geometries
  71. DNA analysis
  72. DNA and RNA quantification
  73. DNA-binding domains (DBD)
  74. DNA profiling
  75. dose–response studies
  76. double barrel coaxial pipette
  77. droplet-in-oil-based isolation
  78. droplet microfluidics
  79. dual-barrel nanopipettes
  80. dual dye and single wavelength intensity ratio measurement
  81. Dynachip Cellectricon
  82. Dynaflow device
  83. Dynaflow platform
  84.  
  85. e
  86. electrochemical (EC) patterning
    1. benefits
    2. 2.5D to 3D shape fabrication
    3. fluidFM probe
    4. glass micro-and nanopipettes
    5. localized electrodeposition
    6. local covalent modification of conductive surfaces
    7. scanning electrochemical cell microscopy (SECCM)
  87. electrochemical push-pull probes
  88. electropermeabilization technique
  89. electroporation
  90. electrospray ionization (ESI)-MS
  91. envelope size and pressure drop
  92. enzymatic activity assays
  93. epithelial-mesenchymal transition (EMT)
  94. external microthermocouple probes
  95. extracellular fluid delivery
  96.  
  97. f
  98. fast local superfusion concept
  99. FCS, see fluorescence correlation spectroscopy (FCS)
  100. feedback mode, SECM
  101. field excitatory postsynaptic potential (fEPSP)
  102. finite-element modeling
  103. five temperature point enzyme activity curves
  104. floating gradient
  105. floating MFP
  106. flow rate ratio
  107. fluid dynamic viscosity
  108. fluidic force microscopy (FluidFM)
    1. AFM based lithography
    2. cloud diameter monitoring
    3. controlled fluid delivery
    4. development
    5. EC-patterning, see electrochemical (EC) patterning
    6. electrochemical 2D patterning and 3D printing
    7. flow in/out
    8. fluorescent tracer
    9. force-controlled nanopipette
    10. hydrodynamic resistance
    11. illustration
    12. injected volumes
    13. input pressure and flow rate
    14. lithography tool
    15. in optical beam detection configuration
    16. patterning nanoparticles
    17. probe holder in chronological order
    18. stiffness
    19. technology
  109. Fluidigm system
  110. fluid mechanics
    1. pressure, velocity distribution, and non-dimensional quantities
    2. shear stress
  111. fluorescence-activated cell sorting (FACS)
  112. fluorescence correlation spectroscopy (FCS)
  113. fluorogenic single cell kinase assay
  114. FluoroMyelin
  115. force-controlled patch-clamp–
  116. formaldehyde
  117. fountain pen
    1. probe
  118. four-aperture MFPs
  119. free-standing Biopen
  120.  
  121. g
  122. genomic analysis
    1. chromosomal characteristics of adherent cells
    2. DNA analysis
    3. liquid handling
    4. operational parameterization
    5. probe incubation and consumption using μFISH
    6. quantitation of DNA in local lysate
    7. spatial multiplexing of probes
  123. glass needle technologies
  124. glass-pipette based push–pull perfusion systems
  125. glutamate receptors type 1 (GluR1)
  126. glyceraldehyde 3-phosphate dehydrogenase (GAPDH) activity
  127. gray-scale lithography
  128. Green's function
  129.  
  130. h
  131. Hele–Shaw approximation
  132. Hele-Shaw cell
  133. Hele-Shaw flow theory
    1. diffusion, see diffusion
    2. model accuracy
    3. Navier–Stokes equation
    4. point sources, round monopoles and square monopoles
    5. stagnation points and hydrodynamic flow confinement zone
    6. velocity potentials for dipoles and quadrupoles
  134. heterocellular stem cell niche engineering
  135. heterogeneity in cells and their microenvironments
  136. hierarchical HFC
    1. dilution measurement
    2. microscale chemistry
    3. microscale deposition, see microscale deposition
    4. minimal dilution of the processing liquid
    5. numerical simulations
    6. recirculation, see recirculation
  137. hierarchical hydrodynamic flow confinement probe (hHFC)
  138. high Péclet number asymptotic solutions
  139. hollow probes
    1. flow
    2. polymer-based AFM cantilevers
    3. silicon-based AFM cantilevers
    4. stiffness
  140. human anti-thyroglobulin antibody (α -TGB)
  141. human fingerprints
  142. hydrodynamically confined compounds
  143. hydrodynamically confined microflows (HCMs)
    1. CFD validation
    2. envelope size and pressure drop
    3. hydrodynamic loads
    4. numerical modeling
    5. pressure, velocity distribution, and non-dimensional quantities
    6. shear stress
  144. hydrodynamically flow confinement concept
  145. hydrodynamic cell traps
  146. hydrodynamic confinement
  147. hydrodynamic flow confinement (HFC)
    1. principle
    2. zone
  148. hydrophobic AnchorChipTM plate
  149.  
  150. i
  151. immersion liquid
  152. immunohistochemical (IHC) analysis
    1. aperture-array probes
    2. microfluidic probes
    3. micro-IHC on human tissue sections
    4. millimeter-scale immunohistochemistry
    5. multiscale surface interactions
    6. probe design and operating conditions
    7. slit-aperture probes
    8. tissue sections
  153. in-channel electrodes
  154. incompressible Navier–Stokes equation
  155. injection flow rate
  156. in-situ single cell proteomics measurement
  157. integrated fluorescein diacetate (FDA)/propidium iodide (PI) membrane integrity assay
  158. intercellular enzymatic processes
  159. intracellular fluid delivery
  160. ion channel activation
  161. ionic current measurements
    1. FluidFM setup
    2. force-controlled patch clamp
    3. SICM
  162.  
  163.  
  164.  
  165. l
  166. lab-on-a-membrane
  167. labs on a chip
  168. lipid analysis in mouse brain
  169. live cell imaging and biological substrates, chemistrode compatibility
  170. local fluorescence in situ hybridization (μFISH)
  171. localized electrochemical deposition (LECD)
  172. local lysis and sample retrieval protocol
  173. local nucleic acid analysis
    1. cell handling
    2. DNA and RNA quantification
    3. genomic analysis, see genomic analysis
    4. heterogeneity in cells and their microenvironments
    5. local lysis and sample retrieval protocol
    6. MFP platform, head and handling
    7. μFISH protocol
    8. microfluidic devices
    9. microfluidic probe
    10. transcriptomic analysis
  174. local superfusion of tissue slices
  175.  
  176. m
  177. MALDI-MS analysis
  178. mass spectrometry (MS)
  179. mass transfer
    1. fluorescence intensity
    2. fluorescent markers and microscopy
    3. hydrophilic substrate
  180. matrix-assisted laser desorption ionization (MALDI)
  181. MEs, see microelectrodes (MEs)
  182. metal anode guided electroplating (MAGE)
  183. MFP-based antibody/antigen assay
  184. microchanneled cantilevers
    1. flow
    2. polymer-based AFM cantilevers
    3. silicon-based AFM cantilevers
    4. stiffness
  185. microelectrodes (MEs)
  186. μFISH platform
  187. μFISH protocol
  188. microfluidic-based biopatterning techniques
  189. microfluidic chemistrode
  190. microfluidic devices
  191. microfluidic probe (MFP)
    1. components
    2. heads
    3. surface processing
  192. microfluidic push–pull probe/device, see electrochemical push–pull probes
  193. microfluidic quadrupole (MQ)
    1. analysis and characterization
    2. application
    3. biochemical concentration gradient assays
    4. concentration gradient measurement
    5. fluid dynamics perspective
    6. flow pattern
    7. hydrodynamic flow confinement
    8. implementation of
    9. neutrophils
    10. principles and configurations
    11. stagnation point hydrodynamic manipulation
    12. stagnation point visualization
  194. microfluidic technology
  195. micro-IHC on human tissue sections
  196. micro-immunohistochemistry (μIHC)
  197. micromanipulation
  198. micropipettes
  199. microscale deposition
    1. convective transport between two laminar flows
    2. patterning proteins on surfaces
    3. protein deposition
  200. Microsystems Technology (MST)
  201. microthermocouples
  202. micro-to picoliter volumes of liquids
  203. micro-total-analysis systems (μTAS)
  204. millimeter-scale immunohistochemistry
  205. minimal dilution
  206. mRNA profiling
  207. MS-coupled sample fractionation techniques
  208. multifunctional pipette
    1. automation
    2. chip concept
    3. collection and integration of assays and sensors
    4. control schematics
    5. device design and function
    6. fabrication
    7. in-channel electrodes
    8. lab-on-a-membrane
    9. materials and fabrication
    10. multiprobe operation
    11. operation
    12. optofluidic thermometer
    13. single cell superfusion
    14. valve-less switching
  209. multiprobe operation
  210. multistage poration/enzyme activity determination experiment
  211. multi-zone deposition
  212.  
  213. n
  214. nanoelectrospray (nESI)
  215. nanoparticle patterning
    1. contact time with constant pressure
    2. dark field microscope
    3. fluorescence image
    4. polystyrene nanoparticles
  216. nanopipettes
    1. application
    2. dual-barrel
    3. intercellular heterogeneity
    4. MALDI-MS analysis
    5. mass spectrometry
    6. probes
    7. segmented flow analysis
    8. segmented flow sampling
    9. sensing platforms
    10. voltage-controlled sample collection
    11. volume and length scale
  217. NanoPortTM
  218. native electrospray
  219. natural/synthetic jelly/soft matrices
  220. Navier–Stokes equation
  221. negative and positive feedback
  222. neutrophils
  223. next-generation sequencing techniques
  224. non-destructive cell manipulations
  225.  
  226. o
  227. off-stoichiometry thiol elastomer (OSTE) materials
  228. omics sequencing throughput
  229. on-line analysis
  230. open-ended microwells
  231. open microfluidic systems
  232. open space microfluidics
  233. open-volume microfluidics
  234. open-volume optofluidic temperature sensing
  235. open volume probes
  236. open volume technology
  237. optical (fluorescence-based) sensing principle
  238. optofluidic thermometer
  239. organotypic brain slices
    1. brain architecture and functions
    2. design of perfusion chamber
    3. in vivo brain structure
    4. micro-perfusion of live dissociated neural cell cultures
    5. microscope setup
    6. open microfluidics
    7. PDMS MFP design
    8. roller drum cultures
  240.  
  241. p
  242. parallel off-line analysis
  243. patch clamp technique
  244. PDMS-based and silicon-based MFP devices
  245. PDMS MFP design
  246. peptide synthesis technology
  247. PET, see polyethylene terephthalate (PET)
  248. photolithography
  249. physical transport processes, chemistrodes
    1. mass transfer
    2. non-dimensional groups
    3. plug coalescence-limited process
  250. pinched HFC
  251. plug coalescence
    1. dynamics
    2. wetting surface
  252. pneumatic membrane valving
  253. poly (dimethyl siloxane) (PDMS)
  254. polyacrylamide (PA) gel
  255. polyethylene terephthalate (PET)
  256. polymer-based hollow probes
  257. pore-forming glycoside digitonin
  258. probe design
  259. processing liquid
  260. propidium iodide (PI)/fluorescence diacetate (FA)
  261.  
  262. q
  263. Quick Stage chamberTM
  264.  
  265. r
  266. razor blade/laser assisted cutting
  267. recirculation
    1. diffusive transport between two laminar flows
    2. small volumes of liquids within a MFP head
  268. Reynold's number
  269. Rhodamine B (RhB)
  270. Rhodamine 6G (Rh6G)
  271. RNA analysis
  272. roller drum cultures
  273.  
  274. s
  275. scanning electrochemical cell microscopy (SECCM)
  276. scanning electrochemical microscopy (SECM)
    1. feedback mode
    2. generation/collection modes
    3. principles of
    4. soft probes, see soft probes
  277. scanning ion conductance microscopy (SICM)
  278. SECM, see scanning electrochemical microscopy (SECM)
  279. segmented flow analysis
  280. segmented flow sampling
  281. selective superfusion
  282. serpentine zones
  283. shear stress
  284. Si-based MFP
  285. silicon-based AFM cantilevers
  286. silicone elastomers
  287. single Allium cepa cell analysis
  288. single-cell adhesion forces
    1. chemical fixation
    2. FluidFM-based single cell force spectroscopy
    3. quantification
  289. single cell analysis
  290. single-cell biology
    1. cell dispensing/removal
    2. cell elasticity
    3. extracellular fluid delivery
    4. FluidFM
      1. cantilevers
      2. patch-clamp
      3. picoampere current measurement
      4. scanning ion conductance microscopy (SICM)
    5. intracellular fluid delivery
    6. molecular analysis
    7. non-destructive cell manipulations
    8. pick-and-place procedure
    9. SCFS
    10. spatial cell manipulation
    11. substrate micropatterning
  291. single-cell electroporation (SCE)
  292. single-cell enzyme activity platform
  293. single cell enzymology
  294. single-cell force spectroscopy (SCFS)
  295. single cell proteomic analysis
    1. applications
    2. assays
    3. cell-to-cell variability
    4. in-situ single cell proteomics measurement
    5. methods
    6. molecular control of cell behavior
    7. next-generation
    8. open-ended microwells
    9. protein expression level
    10. sensitivity
    11. technical requirements
    12. throughput
  296. single cell RNAseq
  297. single cell superfusion
  298. single-cell viability test
  299. single muscle fiber physiology
  300. six channel MFP flow profiles
  301. six channel microfluidic probes
  302. skin biopsy
  303. skin tissue engineering
  304. slit-aperture probes
  305. small model organism
  306. soft linear microelectrode arrays
  307. soft microfluidic SECM probes
    1. approach curves
    2. contact mode scanning
    3. cyclic voltammetry
    4. electrochemical push–pull probe
    5. enzymatic reaction
    6. EPFL logo
    7. feedback mode line scans
    8. fingerprint imaging
    9. flow rate
    10. fluorescence microscopy images
    11. fountain pen probe
    12. imaging of human fingerprints
    13. insulating and conductive substrates
    14. line scans in feedback mode
    15. local manipulation of live cell microenvironments
    16. pushing and pulling flow rates
    17. SECM experiments
  308. soft probes
    1. advantage
    2. cancer biomarkers detection in skin biopsy
    3. fabrication and characterization
    4. high-throughput patterning and surface modification
    5. imaging of extended three-dimensional samples
    6. operation principles
    7. reactivity and topography
    8. surfaces
  309. soft stylus probe
  310. spatial cell manipulation
    1. pick-and-place procedure
    2. positive patterning
    3. substrate micropatterning
    4. subtractive patterning
  311. Spatialyse platform
  312. specific transcriptome modulations
  313. square microfluidic quadrupole
  314. stage II and stage III melanoma, SECM image of
  315. stagnation point hydrodynamic manipulation
  316. stagnation point visualization
  317. staurosporin
  318. stiffness
  319. stimuli plugs
  320. SU-
  321. substrate generation/tip collection (SG/TC) mode
  322. superfusion techniques
  323. surface processing, MFP
  324.  
  325. t
  326. tetramethylrhodamine ethyl ester (TMRE)
  327. 3D COMSOL Multiphysics model
  328. three-dimensional cellular models
  329. tip generation/substrate collection (TG/SC) mode
  330. TIRFM, see total internal reflection fluorescence microscopy (TIRFM)
  331. tissue microarrays (TMAs)
  332. total internal reflection fluorescence microscopy (TIRFM)
  333. transient receptor potential vanilloid 1 (TRPV1)
  334. tumor necrosis factor (TNF)
  335. tunnelling nanotubes (TNTs)
  336.  
  337.  
  338. v
  339. valve-less switching
  340. velocity potentials for dipoles and quadrupoles
  341. vertical MFP
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