-
- a
- accurate temperature determination
- adherent cells
- advanced MFP heads and holders
- advection-diffusion transport equations
- affinity-based proteomic assays
- alkaline phosphatase
- α -amino-3-hydroxy-5-methyl--isoxazolepropionic acid (AMPA)
- α -hemolysin
- amplification-based nucleic acid analysis
- ANSYS CFX
- antibody assays
- antibody barcode arrays
- antigen-specific cell sorting
- anti-progesterone receptor (α -PR)
- aperture-array probes
- aqueous two-phase system (ATPS)
- antibody assays
- bioreagent patterning
- channel-free cell and reagent micropatterning
- cell patterning, see cell patterning
- collagen microgels
- dextran
- phase diagrams
- polyethylene glycol
- polymer solutions
- aspiration flow rate
- AutoMate Scientific
-
- b
- bacterial cells
- biochemical concentration gradient assays
- biopatterning device
- biopatterning methods
- Biopen
- adherent cells
- application space
- cell zeiosis and ion channel activation
- future technology
- hydrodynamic confinement
- intercellular communication
- local superfusion of tissue slices
- local temperature adjustment and measurement
- single cell analysis
- single-cell electroporation
- single cell enzymology
- single-cell viability test
- single muscle fiber physiology
- superfusion techniques
- technology overview
- work on tissue samples
- bioreagent patterning
- blebs
- brain slice culture techniques
- bulky MFP platform
-
- c
- Calcium Green-
- cancer stem cells
- capillary electrophoresis (CE)
- capsaicin receptor
- Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone
- cell blebbing
- cell co-culturing
- cell exclusion and cell island patterning
- cell handling
- cell patterning
- bacterial cells
- cell co-culturing
- cell exclusion and cell island patterning
- heterocellular stem cell niche engineering
- skin tissue engineering
- three-dimensional cellular models
- cell-to-cell variability
- cellular heterogeneity
- cell zeiosis
- chemical single-cell poration
- chemistrodes
- design and fabrication
- geometry and operation
- intracellular processes
- isolation and incubation of cells
- laminar-flow devices
- mass transfer
- non-dimensional groups
- on-line analysis
- operation
- parallel off-line analysis
- plug coalescence-limited process
- single cell stimulation and response analysis
- stimuli plugs
- circulating tumor cell (CTC) sorting
- closed-channel microfluidics
- coaxial double barrel system
- collagen microgels
- computational fluid dynamics (CFD)
- COMSOL Multiphysics
- concentration gradient measurement
- continuous-flow printing
- creeping flow
- CV, see cyclic voltammetry (CV)
- 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX)
- cyclic voltammetry (CV)
- cytosolic free Ca2+
-
- d
- Damköhler number
- Deborah number (De)
- desorption phase
- 3,3'-diaminobenzidine (DAB)
- dichlorodiphenyltrichloroethane (DTT)
- diffusion
- advection-diffusion transport equations
- high Péclet number asymptotic solutions
- model accuracy
- dipolar and quadrupolar MFP geometries
- DNA analysis
- DNA and RNA quantification
- DNA-binding domains (DBD)
- DNA profiling
- dose–response studies
- double barrel coaxial pipette
- droplet-in-oil-based isolation
- droplet microfluidics
- dual-barrel nanopipettes
- dual dye and single wavelength intensity ratio measurement
- Dynachip Cellectricon
- Dynaflow device
- Dynaflow platform
-
- e
- electrochemical (EC) patterning
- benefits
- 2.5D to 3D shape fabrication
- fluidFM probe
- glass micro-and nanopipettes
- localized electrodeposition
- local covalent modification of conductive surfaces
- scanning electrochemical cell microscopy (SECCM)
- electrochemical push-pull probes
- electropermeabilization technique
- electroporation
- electrospray ionization (ESI)-MS
- envelope size and pressure drop
- enzymatic activity assays
- epithelial-mesenchymal transition (EMT)
- external microthermocouple probes
- extracellular fluid delivery
-
- f
- fast local superfusion concept
- FCS, see fluorescence correlation spectroscopy (FCS)
- feedback mode, SECM
- field excitatory postsynaptic potential (fEPSP)
- finite-element modeling
- five temperature point enzyme activity curves
- floating gradient
- floating MFP
- flow rate ratio
- fluid dynamic viscosity
- fluidic force microscopy (FluidFM)
- AFM based lithography
- cloud diameter monitoring
- controlled fluid delivery
- development
- EC-patterning, see electrochemical (EC) patterning
- electrochemical 2D patterning and 3D printing
- flow in/out
- fluorescent tracer
- force-controlled nanopipette
- hydrodynamic resistance
- illustration
- injected volumes
- input pressure and flow rate
- lithography tool
- in optical beam detection configuration
- patterning nanoparticles
- probe holder in chronological order
- stiffness
- technology
- Fluidigm system
- fluid mechanics
- pressure, velocity distribution, and non-dimensional quantities
- shear stress
- fluorescence-activated cell sorting (FACS)
- fluorescence correlation spectroscopy (FCS)
- fluorogenic single cell kinase assay
- FluoroMyelin
- force-controlled patch-clamp–
- formaldehyde
- fountain pen
- probe
- four-aperture MFPs
- free-standing Biopen
-
- g
- genomic analysis
- chromosomal characteristics of adherent cells
- DNA analysis
- liquid handling
- operational parameterization
- probe incubation and consumption using μFISH
- quantitation of DNA in local lysate
- spatial multiplexing of probes
- glass needle technologies
- glass-pipette based push–pull perfusion systems
- glutamate receptors type 1 (GluR1)
- glyceraldehyde 3-phosphate dehydrogenase (GAPDH) activity
- gray-scale lithography
- Green's function
-
- h
- Hele–Shaw approximation
- Hele-Shaw cell
- Hele-Shaw flow theory
- diffusion, see diffusion
- model accuracy
- Navier–Stokes equation
- point sources, round monopoles and square monopoles
- stagnation points and hydrodynamic flow confinement zone
- velocity potentials for dipoles and quadrupoles
- heterocellular stem cell niche engineering
- heterogeneity in cells and their microenvironments
- hierarchical HFC
- dilution measurement
- microscale chemistry
- microscale deposition, see microscale deposition
- minimal dilution of the processing liquid
- numerical simulations
- recirculation, see recirculation
- hierarchical hydrodynamic flow confinement probe (hHFC)
- high Péclet number asymptotic solutions
- hollow probes
- flow
- polymer-based AFM cantilevers
- silicon-based AFM cantilevers
- stiffness
- human anti-thyroglobulin antibody (α -TGB)
- human fingerprints
- hydrodynamically confined compounds
- hydrodynamically confined microflows (HCMs)
- CFD validation
- envelope size and pressure drop
- hydrodynamic loads
- numerical modeling
- pressure, velocity distribution, and non-dimensional quantities
- shear stress
- hydrodynamically flow confinement concept
- hydrodynamic cell traps
- hydrodynamic confinement
- hydrodynamic flow confinement (HFC)
- principle
- zone
- hydrophobic AnchorChipTM plate
-
- i
- immersion liquid
- immunohistochemical (IHC) analysis
- aperture-array probes
- microfluidic probes
- micro-IHC on human tissue sections
- millimeter-scale immunohistochemistry
- multiscale surface interactions
- probe design and operating conditions
- slit-aperture probes
- tissue sections
- in-channel electrodes
- incompressible Navier–Stokes equation
- injection flow rate
- in-situ single cell proteomics measurement
- integrated fluorescein diacetate (FDA)/propidium iodide (PI) membrane integrity assay
- intercellular enzymatic processes
- intracellular fluid delivery
- ion channel activation
- ionic current measurements
- FluidFM setup
- force-controlled patch clamp
- SICM
-
-
-
- l
- lab-on-a-membrane
- labs on a chip
- lipid analysis in mouse brain
- live cell imaging and biological substrates, chemistrode compatibility
- local fluorescence in situ hybridization (μFISH)
- localized electrochemical deposition (LECD)
- local lysis and sample retrieval protocol
- local nucleic acid analysis
- cell handling
- DNA and RNA quantification
- genomic analysis, see genomic analysis
- heterogeneity in cells and their microenvironments
- local lysis and sample retrieval protocol
- MFP platform, head and handling
- μFISH protocol
- microfluidic devices
- microfluidic probe
- transcriptomic analysis
- local superfusion of tissue slices
-
- m
- MALDI-MS analysis
- mass spectrometry (MS)
- mass transfer
- fluorescence intensity
- fluorescent markers and microscopy
- hydrophilic substrate
- matrix-assisted laser desorption ionization (MALDI)
- MEs, see microelectrodes (MEs)
- metal anode guided electroplating (MAGE)
- MFP-based antibody/antigen assay
- microchanneled cantilevers
- flow
- polymer-based AFM cantilevers
- silicon-based AFM cantilevers
- stiffness
- microelectrodes (MEs)
- μFISH platform
- μFISH protocol
- microfluidic-based biopatterning techniques
- microfluidic chemistrode
- microfluidic devices
- microfluidic probe (MFP)
- components
- heads
- surface processing
- microfluidic push–pull probe/device, see electrochemical push–pull probes
- microfluidic quadrupole (MQ)
- analysis and characterization
- application
- biochemical concentration gradient assays
- concentration gradient measurement
- fluid dynamics perspective
- flow pattern
- hydrodynamic flow confinement
- implementation of
- neutrophils
- principles and configurations
- stagnation point hydrodynamic manipulation
- stagnation point visualization
- microfluidic technology
- micro-IHC on human tissue sections
- micro-immunohistochemistry (μIHC)
- micromanipulation
- micropipettes
- microscale deposition
- convective transport between two laminar flows
- patterning proteins on surfaces
- protein deposition
- Microsystems Technology (MST)
- microthermocouples
- micro-to picoliter volumes of liquids
- micro-total-analysis systems (μTAS)
- millimeter-scale immunohistochemistry
- minimal dilution
- mRNA profiling
- MS-coupled sample fractionation techniques
- multifunctional pipette
- automation
- chip concept
- collection and integration of assays and sensors
- control schematics
- device design and function
- fabrication
- in-channel electrodes
- lab-on-a-membrane
- materials and fabrication
- multiprobe operation
- operation
- optofluidic thermometer
- single cell superfusion
- valve-less switching
- multiprobe operation
- multistage poration/enzyme activity determination experiment
- multi-zone deposition
-
- n
- nanoelectrospray (nESI)
- nanoparticle patterning
- contact time with constant pressure
- dark field microscope
- fluorescence image
- polystyrene nanoparticles
- nanopipettes
- application
- dual-barrel
- intercellular heterogeneity
- MALDI-MS analysis
- mass spectrometry
- probes
- segmented flow analysis
- segmented flow sampling
- sensing platforms
- voltage-controlled sample collection
- volume and length scale
- NanoPortTM
- native electrospray
- natural/synthetic jelly/soft matrices
- Navier–Stokes equation
- negative and positive feedback
- neutrophils
- next-generation sequencing techniques
- non-destructive cell manipulations
-
- o
- off-stoichiometry thiol elastomer (OSTE) materials
- omics sequencing throughput
- on-line analysis
- open-ended microwells
- open microfluidic systems
- open space microfluidics
- open-volume microfluidics
- open-volume optofluidic temperature sensing
- open volume probes
- open volume technology
- optical (fluorescence-based) sensing principle
- optofluidic thermometer
- organotypic brain slices
- brain architecture and functions
- design of perfusion chamber
- in vivo brain structure
- micro-perfusion of live dissociated neural cell cultures
- microscope setup
- open microfluidics
- PDMS MFP design
- roller drum cultures
-
- p
- parallel off-line analysis
- patch clamp technique
- PDMS-based and silicon-based MFP devices
- PDMS MFP design
- peptide synthesis technology
- PET, see polyethylene terephthalate (PET)
- photolithography
- physical transport processes, chemistrodes
- mass transfer
- non-dimensional groups
- plug coalescence-limited process
- pinched HFC
- plug coalescence
- dynamics
- wetting surface
- pneumatic membrane valving
- poly (dimethyl siloxane) (PDMS)
- polyacrylamide (PA) gel
- polyethylene terephthalate (PET)
- polymer-based hollow probes
- pore-forming glycoside digitonin
- probe design
- processing liquid
- propidium iodide (PI)/fluorescence diacetate (FA)
-
- q
- Quick Stage chamberTM
-
- r
- razor blade/laser assisted cutting
- recirculation
- diffusive transport between two laminar flows
- small volumes of liquids within a MFP head
- Reynold's number
- Rhodamine B (RhB)
- Rhodamine 6G (Rh6G)
- RNA analysis
- roller drum cultures
-
- s
- scanning electrochemical cell microscopy (SECCM)
- scanning electrochemical microscopy (SECM)
- feedback mode
- generation/collection modes
- principles of
- soft probes, see soft probes
- scanning ion conductance microscopy (SICM)
- SECM, see scanning electrochemical microscopy (SECM)
- segmented flow analysis
- segmented flow sampling
- selective superfusion
- serpentine zones
- shear stress
- Si-based MFP
- silicon-based AFM cantilevers
- silicone elastomers
- single Allium cepa cell analysis
- single-cell adhesion forces
- chemical fixation
- FluidFM-based single cell force spectroscopy
- quantification
- single cell analysis
- single-cell biology
- cell dispensing/removal
- cell elasticity
- extracellular fluid delivery
- FluidFM
- cantilevers
- patch-clamp
- picoampere current measurement
- scanning ion conductance microscopy (SICM)
- intracellular fluid delivery
- molecular analysis
- non-destructive cell manipulations
- pick-and-place procedure
- SCFS
- spatial cell manipulation
- substrate micropatterning
- single-cell electroporation (SCE)
- single-cell enzyme activity platform
- single cell enzymology
- single-cell force spectroscopy (SCFS)
- single cell proteomic analysis
- applications
- assays
- cell-to-cell variability
- in-situ single cell proteomics measurement
- methods
- molecular control of cell behavior
- next-generation
- open-ended microwells
- protein expression level
- sensitivity
- technical requirements
- throughput
- single cell RNAseq
- single cell superfusion
- single-cell viability test
- single muscle fiber physiology
- six channel MFP flow profiles
- six channel microfluidic probes
- skin biopsy
- skin tissue engineering
- slit-aperture probes
- small model organism
- soft linear microelectrode arrays
- soft microfluidic SECM probes
- approach curves
- contact mode scanning
- cyclic voltammetry
- electrochemical push–pull probe
- enzymatic reaction
- EPFL logo
- feedback mode line scans
- fingerprint imaging
- flow rate
- fluorescence microscopy images
- fountain pen probe
- imaging of human fingerprints
- insulating and conductive substrates
- line scans in feedback mode
- local manipulation of live cell microenvironments
- pushing and pulling flow rates
- SECM experiments
- soft probes
- advantage
- cancer biomarkers detection in skin biopsy
- fabrication and characterization
- high-throughput patterning and surface modification
- imaging of extended three-dimensional samples
- operation principles
- reactivity and topography
- surfaces
- soft stylus probe
- spatial cell manipulation
- pick-and-place procedure
- positive patterning
- substrate micropatterning
- subtractive patterning
- Spatialyse platform
- specific transcriptome modulations
- square microfluidic quadrupole
- stage II and stage III melanoma, SECM image of
- stagnation point hydrodynamic manipulation
- stagnation point visualization
- staurosporin
- stiffness
- stimuli plugs
- SU-
- substrate generation/tip collection (SG/TC) mode
- superfusion techniques
- surface processing, MFP
-
- t
- tetramethylrhodamine ethyl ester (TMRE)
- 3D COMSOL Multiphysics model
- three-dimensional cellular models
- tip generation/substrate collection (TG/SC) mode
- TIRFM, see total internal reflection fluorescence microscopy (TIRFM)
- tissue microarrays (TMAs)
- total internal reflection fluorescence microscopy (TIRFM)
- transient receptor potential vanilloid 1 (TRPV1)
- tumor necrosis factor (TNF)
- tunnelling nanotubes (TNTs)
-
-
- v
- valve-less switching
- velocity potentials for dipoles and quadrupoles
- vertical MFP
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