In Python, it is possible to generate file hashes using the hashlib module, as shown here:

$ python
Python 2.7.12 (default, Nov 19 2016, 06:48:10)
>>> import hashlib
>>> content = open(r"log.exe","rb").read()
>>> print hashlib.md5(content).hexdigest()
6e4e030fbd2ee786e1b6b758d5897316
>>> print hashlib.sha256(content).hexdigest()
01636faaae739655bf88b39d21834b7dac923386d2b52efb4142cb278061f97f
>>> print hashlib.sha1(content).hexdigest()
625644bacf83a889038e4a283d29204edc0e9b65